Journal: Biomedical Optics Express

Article Title: 3D-printed biosensors in biomedical applications exploiting plasmonic phenomena and antibody self-assembled monolayers

doi: 10.1364/boe.516565

Figure Lengend Snippet: Fig. 11. (a) SPR spectra obtained by the 3D-printed biosensor for different values of p27 concentration. (b) Absolute value of the experimental variation in resonance wavelength (black marker), calculated with respect to the resonance wavelength of the blank, with relative error bars. The Langmuir fitting (red line) of the experimental values is also reported.

Article Snippet: Human recombinant p27 protein was furnished by OriGene Technologies, Inc. (Rockville, MD, USA).

Techniques: Concentration Assay, Marker

Journal: Biomedical Optics Express

Article Title: 3D-printed biosensors in biomedical applications exploiting plasmonic phenomena and antibody self-assembled monolayers

doi: 10.1364/boe.516565

Figure Lengend Snippet: Fig. 12. (a) Normalized transmitted spectra obtained with the analyte (p27) and two interferents (MMP-2 and IL-6); (b) Experimental resonance wavelength variations (calculated with respect to the blank) for the three analyzed solutions.

Article Snippet: Human recombinant p27 protein was furnished by OriGene Technologies, Inc. (Rockville, MD, USA).

Techniques:

Journal: Antibodies

Article Title: Exploring Parametric and Mechanistic Differences between Expi293F TM and ExpiCHO-S TM Cells for Transient Antibody Production Optimization

doi: 10.3390/antib12030053

Figure Lengend Snippet: Co-transfecting the genes encoding cell cycle inhibitor p21 and p27 enhanced transient expression in Expi293F TM and ExpiCHO-S TM cells, with a bigger effect in ExpiCHO-S TM . As described in , the target DNAs encoding antibody-A, antibody-B, and antibody C complexed with ExpiFectamine™ along with different amounts of plasmid DNAs encoding p21/p27 were transfected into Expi293F TM (Panel A ) and ExpiCHO-S TM (Panel B ) cells. The control titers without p21/p27 DNAs were set as 100% ( n = 3 ± S.D, #, p < 0.05).

Article Snippet: Plasmid DNAs encoding antibody heavy chain (HC) or light chains (LC) were in a cytomegalovirus (CMV)-promoter-based DNA expression vector pTT5. cDNA clones encoding human p21 (pCMV3-p21, Ref Seq number NM_000389.3, CAT#HG11108-UT) and p27 (pCMV3-p27, Ref Seq number NM_004064.3, CAT #HG11109-UT) were purchased from SinoBiological (Wayne, PA, USA).

Techniques: Expressing, Plasmid Preparation, Transfection

Journal: Antibodies

Article Title: Exploring Parametric and Mechanistic Differences between Expi293F TM and ExpiCHO-S TM Cells for Transient Antibody Production Optimization

doi: 10.3390/antib12030053

Figure Lengend Snippet: Optimized parameters for the TGE processes in Expi293F TM and ExpiCHO-S TM cells.

Article Snippet: Plasmid DNAs encoding antibody heavy chain (HC) or light chains (LC) were in a cytomegalovirus (CMV)-promoter-based DNA expression vector pTT5. cDNA clones encoding human p21 (pCMV3-p21, Ref Seq number NM_000389.3, CAT#HG11108-UT) and p27 (pCMV3-p27, Ref Seq number NM_004064.3, CAT #HG11109-UT) were purchased from SinoBiological (Wayne, PA, USA).

Techniques: